Time schedule by speaker > Horkavcová Diana

Simulated body fluid (SBF) buffered by TRIS is the standard solution (ISO23317) for the evaluation of apatite-forming ability of biomaterials used in in vitro test. TRIS buffer speeds up the glass-ceramic dissolution and thus gives the false positive results of an experiment. This work is concerned on the interaction of glass-ceramic scaffolds (Bioglass® derived) with SBF buffered by BES (the other buffer from the Good´s buffers family). The in vitro test was arranged as “static-dynamic” with daily changed (refreshed) SBF solutions. The pH and Si, Ca and P concentration changes in the SBF leachant were analysed by AAS and UV-VIS. Material was characterized by SEM/EDS, XRD, XRF and BET before and after in vitro test. We found that scaffold dissolution was less significant in SBF+BES than in SBF+TRIS (due to Ca ions releasing) and consequently the hydroxyapatite (HAp) growth rate was slower in SBF+BES. Based on these results we can conclude, that the BES affects the glass-ceramic scaffold dissolution less compared with TRIS buffer.